![]() Then click on the Literature (downloads only) to find the manual. 950,00 SCIE-PLAS SCIE-PLAS V20-SDB Semi-Dry Blotter Unit 490,00 Bio-Rad Bio-Rad Electro-Eluter 290,00. On the Trans-Blot SD Semi-Dry Transfer Cell page click on the document tab below the picture of the system. Bio-Rad PowerPac Universal Power Supply 2.775,00. Of protein may be transferred through the membrane and onto the filter paper below. Under the strong fieldsĭeveloped by this apparatus, transfers may not always be quantitative. Recommended to prevent excessive heating during the run. A current limit (3 mA/cm2 for large gels 5.5 mA/cm2 for mini gels) is Bio Rad Mini Trans-Blot System/Western Blotting System For Rapid Blotting Of Mini-Protean Precast And Handcast Gels : Amazon.in: Industrial & Scientific. Large gelsĬan be transferred for 30 minutes to 1 hour at 15–25 V. Blotting Paper, Absorbent Filter, Bio-Rad Extra thick, for Trans-Blot SD Semi-Dry, Trans-Blot and other semi-dry, rapid and wet/tank transfer systems Thick. Transfer mini gels for 15–30 minutes at 10–15 V. Although the Bio-Rad and Novex instructions don't seem to be that different (once again, see below) I think your best bet would be to follow the Novex instructions since they have probably optimized conditions for their apparatus.įrom the Trans-Blot SD Semi-Dry Transfer Cell, Rev C Instruction Manual (Part 1703940) TE77PWR, with built-in power supply, power consumption 0.7 A, AC/DC input 100 - 240 V AC, pkg of 1 ea. ![]() For most proteins, I usually transfer at 15 V for 25 - 30 min as recommended by Bio-Rad (see below). Semi-dry blotter Blotter unit find Sigma-Aldrich-Z340502 MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich. I occasionally use the Bio-Rad Trans-blot SD for my transfers. Bio-Rad Laboratories warrants the Trans-Blot SD semi-dry electrophoretic transfer cell against defects in materials and workmanship for 1 year. The Criterion blotter provides a combination of blotting efficiency and flexibility in an easy-to-use unit.
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